Hydrogenases for Large Scale Deployment of H2 as a Circular Energy Carrier in Industrial Biotechnology Based on Enzymatic Catalysts.
WP1: Screening and Optimization of Hydrogenases
We will identify novel hydrogenases from extreme environments (terrestrial and marine) to expand our enzyme portfolio for bio-based applications. Through protein engineering, we aim to enhance their resilience to physical and chemical stressors and optimize them for scalable, cost-effective production.
WP2: Upscaling of Hydrogenase Production
This work package focuses on designing and evaluating scalable, cost-efficient production routes for hydrogenases. We will develop a pilot-scale process and identify key cost drivers to support future industrial deployment.
WP3: Hydrogenases for Specialty Chemical Production
We will demonstrate the application of NiFe- and FeFe-hydrogenases in energy-efficient biotransformations for synthesizing high-value specialty chemicals. Key targets include oxidative multi-enzyme reactions using hydrogenase-generated H₂O₂ as a clean oxidant.
WP4: Hydrogenases for Commodity Chemical Production
We will develop and optimize hydrogenase-driven biocatalytic pathways for the sustainable synthesis of bulk chemicals. This includes reductive and oxidative cascades with cofactor regeneration and integrated energy recycling to enhance atom and energy efficiency.
WP5: Industrial Assessment of Hydrogenase-Coupled Reactions
This work package evaluates the technical, economic, environmental, and safety performance of hydrogenase-driven processes under industry-relevant conditions. Special focus is given to identifying process bottlenecks and ensuring safe handling of H₂ and H₂/O₂ mixtures.